Myogenesis pp 43-54 | Cite as

Chromatin Immunoprecipitation in Skeletal Myoblasts

  • Vinay Kumar Rao
  • Shilpa Rani Shankar
  • Reshma TanejaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)


Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.

Key words

Myoblasts Chromatin modifiers Histone modifications Epigenetics Transcription 



Work in the R.T. laboratory is supported by grants from the National Medical Research Council.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Vinay Kumar Rao
    • 1
    • 2
  • Shilpa Rani Shankar
    • 1
  • Reshma Taneja
    • 1
    Email author
  1. 1.Department of Physiology, Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore
  2. 2.Temasek Life Sciences LaboratoryNational University of SingaporeSingaporeSingapore

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