Chromatin Immunoprecipitation in Skeletal Myoblasts
Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.
Key wordsMyoblasts Chromatin modifiers Histone modifications Epigenetics Transcription
Work in the R.T. laboratory is supported by grants from the National Medical Research Council.
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