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Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

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Myogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1889))

Abstract

Brown adipocytes dissipate energy through non-shivering thermogenesis mediated by UCP1 protein, hence representing a powerful target to overcome obesity due to energy surplus. However, brown adipocytes are scarce in adult humans, especially in obese subjects, urging the development of novel strategies to boost the number of these thermogenic adipocytes from a therapeutical perspective. In this regard, transdifferentiation of myoblasts into brown adipocytes represents a promising approach. Here, we describe a method that we have recently developed to transdifferentiate myoblasts into brown adipocytes through CRISPR/Cas9-medidated targeting of MyoD, the master myogenic regulatory factor.

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Acknowledgments

We thank Jun Wu and Mary Larimore for mouse colony maintenance and members of Kuang laboratory for valuable comments. Jingjuan Chen and Chao Wang have contributed equally to this work.

Funding

This work was supported by a grant from the US National Institute of Health (R01AR071649), National Natural Science Foundation of China (81471070) and CAMS Innovation Fund for Medical Sciences (2016-I2M-1-012).

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Author notes

  1. Jingjuan Chen and Chao Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Animal Science, Purdue University, West Lafayette, IN, USA

    Jingjuan Chen, Chao Wang & Shihuan Kuang

  2. Center for Cancer Research, Purdue University, West Lafayette, IN, USA

    Shihuan Kuang

Authors
  1. Jingjuan Chen
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  2. Chao Wang
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  3. Shihuan Kuang
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Corresponding author

Correspondence to Shihuan Kuang .

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Editors and Affiliations

  1. Nofima AS, Ã…s, Norway

    Sissel Beate Rønning

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Chen, J., Wang, C., Kuang, S. (2019). Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8897-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8896-9

  • Online ISBN: 978-1-4939-8897-6

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