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Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

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Book cover Myogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1889))

Abstract

Glucose uptake by skeletal muscle is important for metabolic health. Because skeletal muscle is composed of multiple fiber types that have differing metabolic and contractile properties, studying glucose uptake in whole muscle tissue does not elucidate differences at the cellular level. Here, we describe a procedure that enables the measurement of both glucose uptake and fiber type (by myosin heavy chain isoform expression) in individual rat epitrochlearis muscle fibers.

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Acknowledgments

This work was supported by grants from the National Institutes of Health (R01 DK71771 and R01 AG10026). We thank Jim MacKrell and Haiyan Wang for providing valuable suggestions about the manuscript.

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Authors and Affiliations

  1. Muscle Biology Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, USA

    Mark W. Pataky, Edward B. Arias & Gregory D. Cartee

  2. Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA

    Gregory D. Cartee

  3. Institute of Gerontology, University of Michigan, Ann Arbor, MI, USA

    Gregory D. Cartee

Authors
  1. Mark W. Pataky
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  2. Edward B. Arias
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  3. Gregory D. Cartee
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Corresponding author

Correspondence to Gregory D. Cartee .

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Editors and Affiliations

  1. Nofima AS, Ås, Norway

    Sissel Beate Rønning

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Pataky, M.W., Arias, E.B., Cartee, G.D. (2019). Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8897-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8896-9

  • Online ISBN: 978-1-4939-8897-6

  • eBook Packages: Springer Protocols

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