Myogenesis pp 283-300 | Cite as

Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

  • Mark W. Pataky
  • Edward B. Arias
  • Gregory D. CarteeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)


Glucose uptake by skeletal muscle is important for metabolic health. Because skeletal muscle is composed of multiple fiber types that have differing metabolic and contractile properties, studying glucose uptake in whole muscle tissue does not elucidate differences at the cellular level. Here, we describe a procedure that enables the measurement of both glucose uptake and fiber type (by myosin heavy chain isoform expression) in individual rat epitrochlearis muscle fibers.

Key words

Muscle fiber type Myosin heavy chain Glucose transport Glucose uptake SDS-PAGE 



This work was supported by grants from the National Institutes of Health (R01 DK71771 and R01 AG10026). We thank Jim MacKrell and Haiyan Wang for providing valuable suggestions about the manuscript.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mark W. Pataky
    • 1
  • Edward B. Arias
    • 1
  • Gregory D. Cartee
    • 1
    • 2
    • 3
    Email author
  1. 1.Muscle Biology Laboratory, School of KinesiologyUniversity of MichiganAnn ArborUSA
  2. 2.Department of Molecular and Integrative PhysiologyUniversity of MichiganAnn ArborUSA
  3. 3.Institute of GerontologyUniversity of MichiganAnn ArborUSA

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