Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis
Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.
Key wordsDrosophila Indirect flight muscles Tergal depressor of trochanter Muscular dystrophy Myopathy Myogenesis
Research at UNM is supported by grants from NIH/NIGMS: COBRE Center for Evolutionary and Theoretical Immunology (P30GM110907), the NIH (GM061738 and GM124498) and NSF (1518073) to R.M. Cripps.