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Myogenesis pp 255-266 | Cite as

Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis

  • Eva Veiseth-KentEmail author
  • Vibeke Høst
  • Mona E. Pedersen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)

Abstract

This chapter outlines a method for sample preparation for bottom-up proteomics by LC-MSMS analysis of in vitro proliferated bovine primary skeletal muscle cells. The protocol describes the isolation of bovine primary skeletal muscle cells, extraction of proteins, proteolytic digestion of proteins, and desalting of the final peptide samples. The final peptide samples can be analyzed using various LC-MSMS systems after reconstitution in a suitable elution buffer.

Key words

Proteomics Isolation of primary muscle cells Protein extraction Peptide generation LC-MSMS 

Notes

Acknowledgments

This work was supported by a grant from the Foundation for Research levy on Agricultural Products. We thank Ragnhild Stenberg Berg for critical comments and suggestions regarding the book chapter.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Eva Veiseth-Kent
    • 1
    Email author
  • Vibeke Høst
    • 1
  • Mona E. Pedersen
    • 1
  1. 1.Nofima AS, Osloveien 1AasNorway

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