Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis
This chapter outlines a method for sample preparation for bottom-up proteomics by LC-MSMS analysis of in vitro proliferated bovine primary skeletal muscle cells. The protocol describes the isolation of bovine primary skeletal muscle cells, extraction of proteins, proteolytic digestion of proteins, and desalting of the final peptide samples. The final peptide samples can be analyzed using various LC-MSMS systems after reconstitution in a suitable elution buffer.
Key wordsProteomics Isolation of primary muscle cells Protein extraction Peptide generation LC-MSMS
This work was supported by a grant from the Foundation for Research levy on Agricultural Products. We thank Ragnhild Stenberg Berg for critical comments and suggestions regarding the book chapter.
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