Myogenesis pp 213-228 | Cite as

LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue

  • Marta Moreno-Torres
  • Jesper F. Havelund
  • Nils J. FaergemanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)


Liquid chromatography–mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.

Key words

Lipidomics Acyl-CoA Acyl-carnitine Liquid chromatography Mass spectrometry Stable isotope tracers C2C12 myotubes Skeletal muscle tissue Labeling Palmitate 



This work was supported by The Danish Council for Independent Research, Natural Sciences. Marta Moreno-Torres was supported by a postdoctoral grant from The Danish Diabetes Academy.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Marta Moreno-Torres
    • 1
  • Jesper F. Havelund
    • 1
  • Nils J. Faergeman
    • 1
    Email author
  1. 1.Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical SciencesUniversity of Southern DenmarkOdense MDenmark

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