LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue
Liquid chromatography–mass spectrometry (LC-MS) is a widely used methodology for measuring lipids at a global level. Combined with an optimal extraction method LC-MS enables the detection and characterization of a wide range of lipid species even of low abundance. Here, we describe two extraction- and LC-MS-based quantitative analytical methods for lipid, acyl-CoA, and acyl-carnitine analyses from either mouse C2C12 myotubes or mouse skeletal tissue. We also describe the use of 13C16-palmitate and its incorporation into acyl-carnitines to show how stable isotope tracers are metabolized within cells and therefore can be implemented for lipidomic flux analysis.
Key wordsLipidomics Acyl-CoA Acyl-carnitine Liquid chromatography Mass spectrometry Stable isotope tracers C2C12 myotubes Skeletal muscle tissue Labeling Palmitate
This work was supported by The Danish Council for Independent Research, Natural Sciences. Marta Moreno-Torres was supported by a postdoctoral grant from The Danish Diabetes Academy.
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