Myogenesis pp 185-212 | Cite as

Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System

  • Danielle E. Dye
  • Beverley F. Kinnear
  • Vishal Chaturvedi
  • Deirdre R. CoombeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1889)


The ability to grow C2C12 myoblasts in a completely defined, serum free medium enables researchers to investigate the role of specific factors in myoblast proliferation, migration, fusion, and differentiation without the confounding effects of serum. The use of defined, animal free in vitro culture systems will improve reproducibility between research groups and may also enhance translation of tissue engineering techniques into clinical applications. Here, we describe the use and characterization of a serum free culture system for C2C12 myoblasts using standard tissue culture medium and readily available, defined growth factors and supplements.

Key words

C2C12 myoblasts Serum free culture Muscle gene expression 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Danielle E. Dye
    • 1
  • Beverley F. Kinnear
    • 1
  • Vishal Chaturvedi
    • 1
  • Deirdre R. Coombe
    • 1
    Email author
  1. 1.School of Pharmacy and Biomedical Science, Curtin Health Innovation Research Institute, Faculty of Health ScienceCurtin UniversityBentleyAustralia

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