Abstract
Primary myoblasts derived from human tissue are a valuable tool in research of muscle disease and pathophysiology. However, skeletal muscle biopsies, especially from diseased muscle, contain a plethora of non-myogenic cells, necessitating purification of the myogenic cell population. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.
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Acknowledgments
This work is supported by a grant from the Muscular Dystrophy Association #479606 (EG) and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number 1R01AR069582-01 (EG). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. This protocol was modified from previous work, specifically from the listed references [10, 11].
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Pakula, A., Spinazzola, J.M., Gussoni, E. (2019). Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS). In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_1
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DOI: https://doi.org/10.1007/978-1-4939-8897-6_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8896-9
Online ISBN: 978-1-4939-8897-6
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