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Prenatal Diagnosis pp 129–137Cite as

Rapid Prenatal Aneuploidy Screening by Fluorescence In Situ Hybridization (FISH)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1885))

Abstract

The most common aneuploidies observed in prenatal diagnostics in the second trimester are trisomies of the chromosomes 13, 18 or 21 and gonosomal abnormalities. Rapid detection of these aneuploidies after amniocentesis is possible by fluorescence in situ hybridization (FISH) utilizing centromeric or locus-specific probes. FISH aneuploidy screening results in uncultured amniocytes are available within 24 h or less. Operators should be aware that there are possible pitfalls in connection with the commercially available probe sets and in result interpretation in general and thus proceed with appropriate caution. Here, we explain how rapid prenatal aneuploidy screening is performed using the Food and Drug Administration (FDA-) approved Aneu Vysion kit (ABBOTT/Vysis) and a review is given of drawbacks and opportunities of the method.

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Authors and Affiliations

  1. Jena University Hospital, Institute of Human Genetics, Friedrich Schiller University, Jena, Germany

    Anja Weise & Thomas Liehr

Authors
  1. Anja Weise
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  2. Thomas Liehr
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Correspondence to Anja Weise .

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Editors and Affiliations

  1. Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA

    Brynn Levy

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Weise, A., Liehr, T. (2019). Rapid Prenatal Aneuploidy Screening by Fluorescence In Situ Hybridization (FISH). In: Levy, B. (eds) Prenatal Diagnosis. Methods in Molecular Biology, vol 1885. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8889-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8889-1_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8887-7

  • Online ISBN: 978-1-4939-8889-1

  • eBook Packages: Springer Protocols

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