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Quantitative Identification of Senescent Cells in Cancer

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Cancer Immunosurveillance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1884))

Abstract

Cellular senescence, a state of permanent growth arrest, is an important mechanism preventing the propagation of damaged cells. It suppresses cancer development in premalignant lesions in response to activated oncogenes and in tumors following therapy. The presence of senescent cells in premalignant lesions and tumors is controlled by the immune system. The ability to identify and quantify senescent cells more efficiently in vivo is necessary in order to evaluate the effect of these cells on tumorigenesis and cancer therapy. Through combining senescent-associated beta-galactosidase staining with ImageStream X analysis, we have developed an effective method to identify and quantify senescent cancer cells in vivo.

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Acknowledgments

This work was supported by grants to V.K. from the European Research Council under the European Union’s FP7 and from the Israel Science Foundation; and staff scientist internal grant from the Weizmann Institute of Science to Z.P. V.K. is an incumbent of the Karl and Frances Korn Career Development Chair in Life Sciences.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel

    Anat Biran & Valery Krizhanovsky

  2. Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel

    Ziv Porat

Authors
  1. Anat Biran
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  2. Ziv Porat
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  3. Valery Krizhanovsky
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Corresponding authors

Correspondence to Ziv Porat or Valery Krizhanovsky .

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Editors and Affiliations

  1. Departamento de Biología Funcional, Inmunología, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain

    Alejandro López-Soto

  2. Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Asturias, Spain

    Alicia R. Folgueras

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Biran, A., Porat, Z., Krizhanovsky, V. (2019). Quantitative Identification of Senescent Cells in Cancer. In: López-Soto, A., Folgueras, A. (eds) Cancer Immunosurveillance. Methods in Molecular Biology, vol 1884. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8885-3_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8885-3_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8884-6

  • Online ISBN: 978-1-4939-8885-3

  • eBook Packages: Springer Protocols

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