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Evaluating the Metabolic Alterations in Pancreatic Cancer

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Pancreatic Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1882))

Abstract

Metabolic reprograming is an established hallmark of cancer cells. Pancreatic cancer cells, by virtue of the underlying oncogenic drivers, demonstrate metabolic reprograming to sustain growth, invasiveness, and therapy resistance. The increased demands of the growing tumor cells alter the metabolic and signaling pathways to meet the growing nutrient requirements. Investigating the metabolic vulnerabilities of tumor cells can help in developing effective therapeutics to target pancreatic cancer. In this chapter, we explain in detail the methods to evaluate the metabolic changes occurring in the tumor. This includes the glucose/glutamine uptake assays and the measurement of reactive oxygen species, extracellular acidification rate, and oxygen consumption rate in the tumor cells. All these physiological assays help in understanding the metabolic nature of the tumor.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, USA

    Aneesha Dasgupta & Pankaj K. Singh

  2. The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA

    Surendra K. Shukla, Venugopal Gunda, Ryan J. King & Pankaj K. Singh

  3. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA

    Pankaj K. Singh

  4. Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA

    Pankaj K. Singh

Authors
  1. Aneesha Dasgupta
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  2. Surendra K. Shukla
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  3. Venugopal Gunda
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  4. Ryan J. King
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  5. Pankaj K. Singh
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Corresponding author

Correspondence to Pankaj K. Singh .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Gloria H. Su

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Dasgupta, A., Shukla, S.K., Gunda, V., King, R.J., Singh, P.K. (2019). Evaluating the Metabolic Alterations in Pancreatic Cancer. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8879-2_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8878-5

  • Online ISBN: 978-1-4939-8879-2

  • eBook Packages: Springer Protocols

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