Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia
The genomic sequencing of chronic lymphocytic leukemia (CLL) samples has provided exciting new venues for the understanding and treatment of this prevalent disease. This feat is possible thanks to high-throughput sequencing methods, such as Illumina sequencing. The interpretation of these data sources requires not only appropriate software and hardware, but also understanding the biology and technology behind the sequencing process. Here, we provide a primer to understand each step in the analysis of point mutations from whole-genome or whole-exome sequencing experiments of tumor and normal samples.
Key wordsBioinformatics Genomics Cancer Next-generation sequencing Leukemia
We thank J.M.P. Freije and X.S. Puente for helpful comments and advice. The Instituto Universitario de Oncología is supported by Fundación Bancaria Caja de Ahorros de Asturias. V.Q. is supported by Ministerio de Economía y Competitividad and Gobierno del Principado de Asturias, including FEDER funding. C.L.-O. is supported by grants from European Research Council (DeAge, ERC Advanced Grant), Ministerio de Economía y Competitividad, Instituto de Salud Carlos III (RTICC) and Progeria Research Foundation.