Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System
Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.
Key wordsAutophagy Autophagic lysosome reformation Autolysosome In vitro reconstitution Dynamic tubulation Mitochondrial network formation