Autophagy pp 611-619 | Cite as

Triggering Mitophagy with Photosensitizers

  • Cheng-Wei Hsieh
  • Wei Yuan YangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)


One can utilize light illumination to stimulate mitochondrial reactive oxygen species production through the use of mitochondria-specific photosensitizers. By proper tuning of the light dosage, the methodology permits probing of a multitude of mitochondrial damage responses, including mitophagy. This light-controllable trick offers unique opportunities for the investigation of mitophagy—one can spatiotemporally define mitochondrial damage, alter the number of impaired mitochondria, as well as modulate the severity of the mitochondrial injury. This light-activated mitophagy can be adapted not only to single-cell imaging techniques but also to cell population-based biochemical assays.

Key words

Autophagy Mitophagy Photosensitizer MitoTracker Deep Red 



This work was supported by the MOST 104-2628-B-001-001-MY4 research grant from the Ministry of Science and Technology in Taiwan.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Biological ChemistryAcademia SinicaTaipeiTaiwan
  2. 2.Institute of Biochemical Sciences, College of Life SciencesNational Taiwan UniversityTaipeiTaiwan

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