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Autophagy pp 611–619Cite as

Triggering Mitophagy with Photosensitizers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1880))

Abstract

One can utilize light illumination to stimulate mitochondrial reactive oxygen species production through the use of mitochondria-specific photosensitizers. By proper tuning of the light dosage, the methodology permits probing of a multitude of mitochondrial damage responses, including mitophagy. This light-controllable trick offers unique opportunities for the investigation of mitophagy—one can spatiotemporally define mitochondrial damage, alter the number of impaired mitochondria, as well as modulate the severity of the mitochondrial injury. This light-activated mitophagy can be adapted not only to single-cell imaging techniques but also to cell population-based biochemical assays.

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Acknowledgments

This work was supported by the MOST 104-2628-B-001-001-MY4 research grant from the Ministry of Science and Technology in Taiwan.

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Authors and Affiliations

  1. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Cheng-Wei Hsieh & Wei Yuan Yang

  2. Institute of Biochemical Sciences, College of Life Sciences, National Taiwan University, Taipei, Taiwan

    Wei Yuan Yang

Authors
  1. Cheng-Wei Hsieh
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  2. Wei Yuan Yang
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Corresponding author

Correspondence to Wei Yuan Yang .

Editor information

Editors and Affiliations

  1. Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis

  2. Signalling Programme, Babraham Institute, Cambridge, UK

    Oliver Florey

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Hsieh, CW., Yang, W.Y. (2019). Triggering Mitophagy with Photosensitizers. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_40

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  • DOI: https://doi.org/10.1007/978-1-4939-8873-0_40

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8872-3

  • Online ISBN: 978-1-4939-8873-0

  • eBook Packages: Springer Protocols

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