Autophagy pp 555-560 | Cite as

Characterization of the “Autophagic Flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy

  • D. Kalamida
  • A. Giatromanolaki
  • M. I. KoukourakisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)


A method is described for the immunostaining of human prostate cancer biopsies for the autophagic marker LC3 and the lysosomal protein LAMP2A. Using this combination we can provide some information on autophagic flux, and specific patterns of staining have been recognized for normal prostate tissue and prostatic carcinomas.

Key words

LC3 Prostate cancer Immunostaining Autophagic flux 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • D. Kalamida
    • 1
  • A. Giatromanolaki
    • 2
  • M. I. Koukourakis
    • 1
    Email author
  1. 1.Department of Radiotherapy/OncologyDemocritus University of Thrace, and University General Hospital of AlexandroupolisAlexandroupolisGreece
  2. 2.Department of PathologyDemocritus University of Thrace, and University General Hospital of AlexandroupolisAlexandroupolisGreece

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