Identification and Validation of Novel Autophagy Regulators Using an Endogenous Readout siGENOME Screen
Autophagy is a highly regulated process, and its deregulation can contribute to various diseases, including cancer, immune diseases, and neurodegenerative disorders. Here we describe the design, protocol, and analysis of an imaging-based high-throughput screen with an endogenous autophagy readout. The screen uses a genome-wide siRNA library to identify autophagy regulators in mammalian cells.
Key wordssiGENOME screen Autophagy Autophagy regulation Endogenous readout LC3 puncta Screen analysis Large data set analysis Hit identification