Autophagy pp 17-56 | Cite as

Structural Studies of Autophagy-Related Proteins

  • Melanie Schwarten
  • Oliver H. Weiergräber
  • Dušan Petrović
  • Birgit Strodel
  • Dieter WillboldEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1880)


Information about the structure and dynamics of proteins is crucial for understanding their physiological functions as well as for the development of strategies to modulate these activities. In this chapter we will describe the work packages required to determine the three-dimensional structures of proteins involved in autophagy by using X-ray crystallography or nuclear magnetic resonance (NMR) spectroscopy. Further we will provide instructions how to perform a molecular dynamics (MD) simulation using GABARAP as example protein.

Key words

X-ray crystallography NMR spectroscopy MD simulation 



The authors acknowledge funding by the Deutsche Forschungsgemeinschaft (SFB1208 and SFB974) and the Jürgen Manchot Stiftung.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Melanie Schwarten
    • 1
  • Oliver H. Weiergräber
    • 1
  • Dušan Petrović
    • 4
    • 1
  • Birgit Strodel
    • 1
    • 2
  • Dieter Willbold
    • 1
    • 3
    Email author
  1. 1.Institute of Complex Systems ICS-6 (Structural Biochemistry)Forschungszentrum JülichJülichGermany
  2. 2.Institute of Theoretical and Computational ChemistryHeinrich Heine University DüsseldorfDüsseldorfGermany
  3. 3.Institut für Physikalische BiologieHeinrich Heine University DüsseldorfDüsseldorfGermany
  4. 4.Department of ChemistryBMC, Uppsala UniversityUppsalaSweden

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