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Phytoplasmas pp 83–95Cite as

Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1875))

Abstract

Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.

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Author information

Authors and Affiliations

  1. Phytobacteriology Laboratory, DISTAL, Alma Mater Studiorum, University of Bologna, Bologna, Italy

    Assunta Bertaccini, Samanta Paltrinieri & Nicoletta Contaldo

Authors
  1. Assunta Bertaccini
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  2. Samanta Paltrinieri
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  3. Nicoletta Contaldo
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Corresponding author

Correspondence to Assunta Bertaccini .

Editor information

Editors and Affiliations

  1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Rita Musetti

  2. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Laura Pagliari

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Bertaccini, A., Paltrinieri, S., Contaldo, N. (2019). Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene. In: Musetti, R., Pagliari, L. (eds) Phytoplasmas. Methods in Molecular Biology, vol 1875. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8837-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8837-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8836-5

  • Online ISBN: 978-1-4939-8837-2

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