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Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas

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Phytoplasmas

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1875))

Abstract

To visualize phytoplasmas at early stages of vector infection, an immunofluorescence assay was developed. The chapter provides experimental details on dissection of salivary glands, incubation of the dissected organs with phytoplasma suspension, fixation, embedding, sectioning, labeling, and final visualization with confocal microscopy. All the procedure will be described for the leafhopper Euscelidius variegatus, natural vector of “Candidatus phytoplasma asteris” and laboratory vector of Flavescence dorée phytoplasma.

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Author information

Authors and Affiliations

  1. Istituto per la Protezione Sostenibile delle Piante, CNR, Torino, Italy

    Luciana Galetto, Marta Vallino & Cristina Marzachì

  2. Citrus Experimental Station, University of Florida, Lake Alfred, FL, USA

    Mahnaz Rashidi

Authors
  1. Luciana Galetto
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  2. Marta Vallino
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  3. Mahnaz Rashidi
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  4. Cristina Marzachì
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Corresponding author

Correspondence to Cristina Marzachì .

Editor information

Editors and Affiliations

  1. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Rita Musetti

  2. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy

    Laura Pagliari

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Galetto, L., Vallino, M., Rashidi, M., Marzachì, C. (2019). Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas. In: Musetti, R., Pagliari, L. (eds) Phytoplasmas. Methods in Molecular Biology, vol 1875. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8837-2_23

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  • DOI: https://doi.org/10.1007/978-1-4939-8837-2_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8836-5

  • Online ISBN: 978-1-4939-8837-2

  • eBook Packages: Springer Protocols

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