DAPI and Confocal Laser-Scanning Microscopy for In Vivo Imaging of Phytoplasmas
As phytoplasmas are located inside the phloem tissue, always surrounded by numerous layers of other cells, they can result difficult candidates for microscopical investigations. Moreover, the necessity to kill the plant tissues for microscopy observations causes instantaneous and irreversible modifications in the sieve elements, leading to misleading information and erroneous interpretations. Phytoplasmas were here investigated in intact Vicia faba host plants using DAPI as fluorescent probe and confocal laser scanning microscopy. The described nondestructive technique may be applied for the imaging of phytoplasmas and of different pathogen-related responses in planta.
Key wordsConfocal laser scanning microscopy DAPI Phytoplasmas Vicia faba
This work was funded with the support of the Deutscher Akademischer Austauschdienst (DAAD) (grant number A/11/04104 for R. Musetti).
The authors are grateful to Elena Zaghini for drawing Fig. 2.
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