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Phytoplasmas pp 239-251 | Cite as

Transcriptomic Analyses of Phytoplasmas

  • Davide Pacifico
  • Simona Abbà
  • Sabrina Palmano
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1875)

Abstract

Transcriptomic analyses addressed to study phytoplasma gene expression may present few difficulties due to the uncultivable nature of these intracellular, obligate pathogens. While RNA extraction from insect vectors does not imply any particular adaptation of the protocols used in most commercial kits, RNA isolation from phytoplasma-infected plants can be a challenging task, given the high levels of polyphenol contents and accumulation of sucrose and starch in the different plant tissues. Here, we describe two different transcriptomic approaches, one focused on RNA phytoplasma sequencing and the other on phytoplasma quantitative gene expression in relation to pathogen load.

Key words

RNA sequencing Quantitative RT-PCR Gene expression Phytoplasma load Expression index 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Davide Pacifico
    • 1
  • Simona Abbà
    • 2
  • Sabrina Palmano
    • 2
  1. 1.Institute of Biosciences and Bioresources, CNRPalermoItaly
  2. 2.Institute for Sustainable Plant Protection, CNRTorinoItaly

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