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Microinjection pp 87–98Cite as

Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1874))

Abstract

Mouse spermatogonial stem cells (SSCs) can be grown in culture for long periods. Cultured SSCs, also called germline stem (GS) cells, maintain themselves by self-renewing proliferation while retaining the ability to differentiate into sperm. Thus, when transplanted into the seminiferous tubules of a host mouse testis, they settle in the basal compartment of the tubules and establish spermatogenenic colonies. The sperm produced in the host are competent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation.

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Acknowledgments

We thank Anri Miyamoto and Kumiko Katagiri for technical assistance. This study was funded by a Grant-in-Aid for Young Scientists (A) 26713012 (to T. S.); The Uehara Memorial Foundation (to T.S.); and Innovative Areas, “Mechanisms regulating gamete formation in animals” 25114007 (to T.O.).

Author information

Authors and Affiliations

  1. Laboratory of Biopharmaceutical and Regenerative Sciences, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science, Yokohama, Kanagawa, Japan

    Takuya Sato & Takehiko Ogawa

  2. Department of Urology, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan

    Takehiko Ogawa

Authors
  1. Takuya Sato
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  2. Takehiko Ogawa
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Corresponding author

Correspondence to Takuya Sato .

Editor information

Editors and Affiliations

  1. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Chengyu Liu

  2. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Yubin Du

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Sato, T., Ogawa, T. (2019). Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method. In: Liu, C., Du, Y. (eds) Microinjection. Methods in Molecular Biology, vol 1874. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8831-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8831-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8830-3

  • Online ISBN: 978-1-4939-8831-0

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