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Quantitation of Tapentadol by Liquid Chromatography: Tandem Mass Spectrometry

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LC-MS in Drug Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1872))

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Abstract

Tapentadol is an orally active analgesic with a similar structure to tramadol. Its primary mechanism of action is agonist action on the mu-opioid receptor. The method described here quantitates tapentadol in the whole blood using a matching deuterated internal standard, extraction via a protein “crash” with acetonitrile, followed by analysis using liquid chromatography-tandem mass spectrometry.

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References

  1. Baselt RC (2017) Disposition of toxic drugs and chemicals in man, 11th edn. Biomedical Publications, Seal Beach, CA

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  2. Ortho-McNeil-Janssen (2010) Nucynta (tapentadol) prescribing information. PriCara Division, Raritan, NJ

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Author information

Authors and Affiliations

  1. Alberta Office of the Chief Medical Examiner, Edmonton, AB, Canada

    Graham R. Jones

  2. Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada

    Russell P. Handy

Authors
  1. Graham R. Jones
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  2. Russell P. Handy
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Corresponding author

Correspondence to Graham R. Jones .

Editor information

Editors and Affiliations

  1. Department of Laboratory, Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Loralie J. Langman

  2. Department of Laboratory, Medicine and Pathology, Mayo Clinic in Arizona, Scottsdale, AZ, USA

    Christine L.H. Snozek

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Jones, G.R., Handy, R.P. (2019). Quantitation of Tapentadol by Liquid Chromatography: Tandem Mass Spectrometry. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 1872. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8823-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8823-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8822-8

  • Online ISBN: 978-1-4939-8823-5

  • eBook Packages: Springer Protocols

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