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Functional Proteomics pp 437–443Cite as

The Use of Proteomics Studies in Identifying Moonlighting Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1871))

Abstract

Proteomics studies that characterize hundreds or thousands of proteins in parallel can play an important part in the identification of moonlighting proteins, proteins that perform two or more distinct and physiologically relevant biochemical or biophysical functions. Functional assays, including ligand-binding assays, can find a surprising second function for a protein that was previously identified as performing a different function, for example, a DNA-binding ability for an enzyme in amino acid metabolism. The results of large-scale assays of protein–protein interactions, gene knockouts, or subcellular protein localizations, or bioinformatics analysis of amino acid sequences and three-dimensional structures, can also be used to predict that a protein has additional functions, but in these cases it is important to use biochemical and biophysical methods to confirm the protein can perform each function.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA

    Constance Jeffery

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  1. Constance Jeffery
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Correspondence to Constance Jeffery .

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Editors and Affiliations

  1. Array Bridge Inc., St. Louis, MO, USA

    Xing Wang

  2. Biotech Support Group LLC, Monmouth Junction, NJ, USA

    Matthew Kuruc

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Jeffery, C. (2019). The Use of Proteomics Studies in Identifying Moonlighting Proteins. In: Wang, X., Kuruc, M. (eds) Functional Proteomics. Methods in Molecular Biology, vol 1871. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8814-3_25

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  • DOI: https://doi.org/10.1007/978-1-4939-8814-3_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8813-6

  • Online ISBN: 978-1-4939-8814-3

  • eBook Packages: Springer Protocols

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