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Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine

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Book cover Epitranscriptomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1870))

Abstract

A powerful method to determine the methylation status of specific cytosine residues within RNA is bisulfite sequencing. In combination with high-throughput sequencing methods cytosine methylation can be determined at nucleotide resolution on a transcriptome-wide level. Nevertheless, several critical aspects need to be considered before starting such a project. Below we describe a detailed step-by-step protocol for planning and performing a transcriptome-wide bisulfite sequencing experiment and subsequent data analysis to determine methyl-cytosine in poly(A)RNA from cells and tissues.

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Acknowledgement

Funding in A.L.’s lab is provided by the Austrian Science Fund (FWF): P27024-BBL.

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Authors and Affiliations

  1. Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

    Lukas Trixl, Thomas Amort & Alexandra Lusser

  2. Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

    Dietmar Rieder

Authors
  1. Lukas Trixl
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  2. Dietmar Rieder
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  3. Thomas Amort
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  4. Alexandra Lusser
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Corresponding author

Correspondence to Alexandra Lusser .

Editor information

Editors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Narendra Wajapeyee

  2. Yale Cancer Center, Yale University School of Medicine, New Haven, CT, USA

    Romi Gupta

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Trixl, L., Rieder, D., Amort, T., Lusser, A. (2019). Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine. In: Wajapeyee, N., Gupta, R. (eds) Epitranscriptomics. Methods in Molecular Biology, vol 1870. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8808-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8808-2_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8807-5

  • Online ISBN: 978-1-4939-8808-2

  • eBook Packages: Springer Protocols

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