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Detecting Stem Cell Marker Expression Using the NanoString nCounter System

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Brain Tumor Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1869))

Abstract

The use of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) has become commonplace in the study of neuronal development, physiology, disease modelling, and therapy development. Due to the transient nature of working with these cells, it is important to regularly confirm the cell status as a naive stem cell versus a more defined neural progenitor cell (NPC). Classically, this has been done using a panel of specific antibodies to test for the expression of transcription factors known to be observed in ESCs, but not NPCs. However, this method is both time consuming and expensive. Here, we describe the use of the NanoString nCounter system for determining the levels of expression of key transcription factors that will effectively aid in determining the state of your stem cell cultures.

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Acknowledgments

Funding support from the Canadian Institute of Health Research (CIHR) Graduate Scholarship (S.R.).

Author information

Authors and Affiliations

  1. Division of Cell Biology, Hospital for Sick Children Research Center, Toronto, ON, Canada

    Scott Ryall, Javal Sheth & Cynthia Hawkins

  2. Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada

    Scott Ryall & Cynthia Hawkins

  3. Department of Pediatric Laboratory Medicine, Hospital for Sick Children, Toronto, ON, Canada

    Anthony Arnoldo, Javal Sheth & Cynthia Hawkins

  4. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada

    Sheila K. Singh

  5. Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada

    Sheila K. Singh

  6. Department of Surgery, McMaster University, Hamilton, ON, Canada

    Sheila K. Singh

Authors
  1. Scott Ryall
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  2. Anthony Arnoldo
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  3. Javal Sheth
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  4. Sheila K. Singh
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  5. Cynthia Hawkins
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Corresponding author

Correspondence to Cynthia Hawkins .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada

    Sheila K. Singh

  2. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada

    Chitra Venugopal

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Ryall, S., Arnoldo, A., Sheth, J., Singh, S.K., Hawkins, C. (2019). Detecting Stem Cell Marker Expression Using the NanoString nCounter System. In: Singh, S., Venugopal, C. (eds) Brain Tumor Stem Cells. Methods in Molecular Biology, vol 1869. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8805-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8805-1_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8804-4

  • Online ISBN: 978-1-4939-8805-1

  • eBook Packages: Springer Protocols

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