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Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain

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Brain Tumor Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1869))

Abstract

Cellular magnetic resonance imaging (MRI) enables visualization of cells in vivo. This is accomplished by labeling cells with superparamagnetic iron oxide nanoparticles. Here, we describe the steps for labeling human cancer cells with iron for tracking them after injection into nude mice. We also provide details for validation of cell labeling, ultrasound guided intra-cardiac injection, and MRI.

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Author information

Authors and Affiliations

  1. Robarts Research Institute, Western University, London, ON, Canada

    Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton & Paula J. Foster

Authors
  1. Katie M. Parkins
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  2. Ashley V. Makela
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  3. Amanda M. Hamilton
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  4. Paula J. Foster
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Corresponding author

Correspondence to Paula J. Foster .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada

    Sheila K. Singh

  2. Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON, Canada

    Chitra Venugopal

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Parkins, K.M., Makela, A.V., Hamilton, A.M., Foster, P.J. (2019). Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain. In: Singh, S., Venugopal, C. (eds) Brain Tumor Stem Cells. Methods in Molecular Biology, vol 1869. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8805-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8805-1_20

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8804-4

  • Online ISBN: 978-1-4939-8805-1

  • eBook Packages: Springer Protocols

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