Abstract
Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.
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References
Ishiguro A, Akiyama T, Adachi H, Inoue J, Nakamura Y (2011) Therapeutic potential of anti-interleukin-17A aptamer: suppression of interleukin-17A signaling and attenuation of autoimmunity in two mouse models. Arthritis Rheum 63:455–466
Li W, Lan X (2015) Aptamer oligonucleotides: novel potential therapeutic agents in autoimmune disease. Nucleic Acid Ther 25:173–179
Hirota M, Murakami I, Ishikawa Y, Suzuki T, Sumida S, Ibaragi S et al (2016) Chemically modified interleukin-6 aptamer inhibits development of collagen-induced arthritis in cynomolgus monkeys. Nucleic Acid Ther 26:10–19
Ellington AD, Szostak JW (1990) In vitro selection of RNA molecules that bind specific ligands. Nature 346:818–822
Tuerk C, Gold L (1990) Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249:505–510
Takahashi M (2018) Aptamers targeting cell surface proteins. Biochimie 145:63–72
Ohuchi S (2012) Cell-SELEX technology. Biores Open Access 1:265–272
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Liu, S., Suzuki, Y., Inui, M. (2018). Generation of Specific Aptamers. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 1868. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8802-0_11
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DOI: https://doi.org/10.1007/978-1-4939-8802-0_11
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8801-3
Online ISBN: 978-1-4939-8802-0
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