Abstract
The electrophoresis on cellulose acetate membrane is most widely used because of its simplicity, and is without the use of any sophisticated instrument other than electrophoresis apparatus and the cellulose acetate strip. Here we describe a modified version of cellulose acetate membrane electrophoresis for hemoglobin separation from blood sample. Sharp, clear bands without tailing effects can be obtained with this method. The method and apparatus described here would be appropriate to separate protein fractions under 1 h at voltages up to 60 V/cm measured between the electrodes.
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Acknowledgment
This work was supported by NIH grant 5RO1AI104944-04 to W.A.D.
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Kumar, R., Derbigny, W.A. (2019). Cellulose Acetate Electrophoresis of Hemoglobin. In: Kurien, B., Scofield, R. (eds) Electrophoretic Separation of Proteins. Methods in Molecular Biology, vol 1855. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8793-1_7
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DOI: https://doi.org/10.1007/978-1-4939-8793-1_7
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