Abstract
Aggregation of beta-amyloid peptides especially Aβ1-42 in amyloid plaques is one of the major neuropathological events in Alzheimer’s disease. This event is normally accompanied by a relative reduction of the concentration of Aβ1-42 in the cerebrospinal fluid (CSF) of patient developing the signs of Alzheimer’s disease. Here, we describe methods for isolation and for microchip gel electrophoresis of Aβ peptides in polydimethylsiloxane (PDMS) microfluidic chip. The method was applied to compare the relative concentration of Aβ1-42 with other Aβ peptides, for example, Aβ 1-40 in CSF. In order to increase the sensitivity of detection, Aβ peptides in the CSF samples were first captured and concentrated using magnetic beads coated with specific anti-Aβ antibodies.
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Acknowledgments
This work was supported by European grants LSHB CT 06 037953 (NeuroTAS) and FP7-NMP-09-246513 (NADINE).
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Mohamadi, M.R., Verpillot, R., Taverna, M., Otto, M., Viovy, JL. (2019). “Microchip Electrophoresis,” with Respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”. In: Kurien, B., Scofield, R. (eds) Electrophoretic Separation of Proteins. Methods in Molecular Biology, vol 1855. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8793-1_28
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DOI: https://doi.org/10.1007/978-1-4939-8793-1_28
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