Abstract
Electroporation allows targeting of genetic materials (e.g., DNA, RNA, antisense morpholinos) to the tissue of interest with high spatial and temporal specificity. Here, we describe a highly efficient and reproducible electroporation strategy for targeting the central nervous system in Xenopus. This versatile approach can be combined with live imaging or other existing experimental procedures to aid the investigation of different research questions.
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Acknowledgment
We thank R. Cagnetta and M. Eldred for critical reading of the manuscript. This work was supported by Cambridge Trust, Croucher Foundation, Sir Edward Youde Memorial Fund (H.H.W), Wellcome Trust Programme Grant (085314/Z/08/Z), and ERC Advanced Grant (322817) (C.E.H).
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Wong, H.HW., Holt, C.E. (2018). Targeted Electroporation in the CNS in Xenopus Embryos. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_9
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DOI: https://doi.org/10.1007/978-1-4939-8784-9_9
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