Abstract
The Xenopus embryonic epidermis serves as a model to investigate the development, cell biology, and regeneration of vertebrate mucociliary epithelia. Its fast development as well as the ease of manipulation and analysis in this system facilitate novel approaches and sophisticated experiments addressing the principle mechanisms of mucociliary signaling, transcriptional regulation, and morphogenesis. This protocol describes how cell type composition can be manipulated and analyzed, and how mucociliary organoids can be generated and used for “omics”-type of experiments.
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Acknowledgment
PW and the Walentek lab are funded by the Deutsche Forschungsgemeinschaft (DFG) through the Emmy Noether Programme grant WA 3365/2-1. PW thanks the Xenopus community and resources for continuous support of his research work, in especially the National Xenopus Resource (RRID:SCR_013731) and Xenbase (RRID:SCR_003280).
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Walentek, P. (2018). Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis. In: Vleminckx, K. (eds) Xenopus. Methods in Molecular Biology, vol 1865. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8784-9_18
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DOI: https://doi.org/10.1007/978-1-4939-8784-9_18
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