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Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato

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Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

Potato is considered the fourth most important food crop in the world, and the most important non-cereal crop. Potato is transformed using Agrobacterium tumefaciens with relative ease. Several improvements have been made in the last 20 years with respect to tissue culture, transformation, and regeneration of potato. This chapter describes a reliable and efficient potato transformation system using internodal explants. Plasmid preparation, Agrobacterium transformation, potato transformation, regeneration, and recovery are described in detail, as well as molecular characterization of resulting putative transgenic plants.

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References

  1. Halterman D, Guenthner J, Collinge S, Butler N, Douches D (2016) Biotech potatoes in the 21st century: 20 years since the first biotech potato. Am J Potato Res 93(1):1–20

    Article  CAS  Google Scholar 

  2. Yoshida K, Schuenemann VJ, Cano LM, Pais M, Mishra B, Sharma R, Lanz C, Martin FN, Kamoun S, Krause J, Thines M, Weigel D, Burbano HA (2013) The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine. elife 2:e00731. https://doi.org/10.7554/eLife.00731

    Article  PubMed  PubMed Central  Google Scholar 

  3. Ooms G, Bossen M, Burrell M, Karp A (1986) Genetic manipulation in potato with Agrobacterium rhizogenes. Potato Res 29(3):367–379

    Article  CAS  Google Scholar 

  4. Stiekema WJ, Heidekamp F, Louwerse JD, Verhoeven HA, Dijkhuis P (1988) Introduction of foreign genes into potato cultivars Bintje and Desiree using an Agrobacterium tumefaciens binary vector. Plant Cell Rep 7(1):47–50

    Article  CAS  Google Scholar 

  5. De Block M (1988) Genotype-independent leaf disc transformation of potato (Solanum tuberosum) using Agrobacterium tumefaciens. Theor Appl Genet 76(5):767–774

    Article  Google Scholar 

  6. Visser RGF, Jacobsen E, Hesseling-Meinders A, Schans MJ, Witholt B, Feenstra WJ (1989) Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments. Plant Mol Biol 12(3):329–337. https://doi.org/10.1007/bf00043210

    Article  CAS  PubMed  Google Scholar 

  7. Heeres P, Schippers-Rozenboom M, Jacobsen E, Visser RGF (2002) Transformation of a large number of potato varieties: genotype-dependent variation in efficiency and somaclonal variability. Euphytica 124(1):13–22. https://doi.org/10.1023/a:1015689112703

    Article  CAS  Google Scholar 

  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  9. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158. https://doi.org/10.1016/0014-4827(68)90403-5

    Article  CAS  PubMed  Google Scholar 

  10. Matsuda F, Yamada T, Miyazawa H, Miyagawa H, Wakasa K (2005) Characterization of tryptophan-overproducing potato transgenic for a mutant rice anthranilate synthase α-subunit gene (OASA1D). Planta 222(3):535–545. https://doi.org/10.1007/s00425-005-1565-x

    Article  CAS  PubMed  Google Scholar 

  11. Miki D, Shimamoto K (2004) Simple RNAi vectors for stable and transient suppression of gene function in rice. Plant Cell Physiol 45(4). doi:https://doi.org/10.1093/pcp/pch048

    Article  CAS  Google Scholar 

  12. Höfgen R, Willmitzer L (1988) Storage of competent cells for Agrobacterium transformation. Nucleic Acids Res 16(20):9877

    Article  Google Scholar 

  13. Chronis D, Chen S, Lang P, Tran T, Thurston D, Wang X (2014) Potato transformation. bio-Protocol 4(1). doi:https://doi.org/10.21769/BioProtoc.1017

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Acknowledgments

A sincere thank you to Whitney Harchenko, for providing photos of her work in transforming potato.

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Authors and Affiliations

  1. Montana State University, Bozeman, MT, USA

    Myron A. Bruce & Jessica L. Shoup Rupp

  2. Kansas State University, Manhattan, KS, USA

    Myron A. Bruce & Jessica L. Shoup Rupp

Authors
  1. Myron A. Bruce
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  2. Jessica L. Shoup Rupp
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Corresponding author

Correspondence to Jessica L. Shoup Rupp .

Editor information

Editors and Affiliations

  1. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Michelle Smith

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Bruce, M.A., Shoup Rupp, J.L. (2019). Agrobacterium-Mediated Transformation of Solanum tuberosum L., Potato. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

  • eBook Packages: Springer Protocols

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