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Genetic Modification of Grapevine Embryogenic Cultures

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Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

Precision breeding is an approach to grapevine genetic improvement that transfers only specific traits among sexually compatible species via the relatively stable mitotic cell division pathway in order to avoid the significant disruption imposed upon conventional breeding by meiosis. Factors enabling precision breeding include the availability of the Vitis genome sequence combined with highly optimized gene insertion and plant regeneration protocols. A protocol for the production of grapevine embryogenic cultures and their genetic transformation is described. Embryogenic cultures are produced from either leaf or floral explants. Somatic embryos at the cotyledonary stage of development are used for Agrobacterium-mediated transformation. Following co-cultivation with Agrobacterium containing the genes of interest, modified embryos are selected on the basis of anthocyanin pigmentation and antibiotic resistance. Somatic embryos are then germinated to produce modified plants that are hardened and transferred to a greenhouse. The presence of the genes of interest is confirmed by PCR.

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Acknowledgments

S. A. Dhekney holds the E.A Whitney Endowed Professorship in the Department of Plant Sciences. M. Brungart-Rosenberg and C. Claflin were supported in part by a grant from the National Institute of General Medical Sciences (2P20GM103432) from the National Institute of Health. The research was supported in part by the Wyoming Agricultural Experiment Station and grants from the USDA/USAID US-Egypt Science and Technology Development Funds program (Grant No. 0210-22310-005-24G).

Author information

Authors and Affiliations

  1. Department of Plant Sciences, Sheridan Research and Extension Center, University of Wyoming, Sheridan, WY, USA

    S. A. Dhekney, S. K. Sessions & M. Brungart-Rosenberg

  2. Sheridan College, Sheridan, WY, USA

    C. Claflin

  3. USDA-ARS, Kearneysville, WV, USA

    Z. T. Li

  4. Mid-Florida Research and Education Center, University of Florida/IFAS, Apopka, FL, USA

    D. J. Gray

Authors
  1. S. A. Dhekney
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  2. S. K. Sessions
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  3. M. Brungart-Rosenberg
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  4. C. Claflin
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  5. Z. T. Li
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  6. D. J. Gray
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Corresponding author

Correspondence to S. A. Dhekney .

Editor information

Editors and Affiliations

  1. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Michelle Smith

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Dhekney, S.A., Sessions, S.K., Brungart-Rosenberg, M., Claflin, C., Li, Z.T., Gray, D.J. (2019). Genetic Modification of Grapevine Embryogenic Cultures. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

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