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Long Range Sequencing and Validation of Insect Genome Assemblies

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Insect Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1858))

Abstract

Advances in long read and long range sequencing technologies have enabled chromosome length resolution for de novo genome assemblies even in the absence of complementary resources such as physical maps. Herein, I introduce a few methods for quality control and discuss potential pitfalls when assembling insect genomes with long reads.

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Acknowledgments

I would like to thank Susan Brown and Michael Pfrender for the invitation to author this article. I would also like to thank my colleagues Prashant Hosmani and Mirella Flores for insightful discussions for troubleshooting assembly issues. This work was funded by USDA NIFA grant 2015-70016-23028 funded to Susan Brown and Lukas Mueller.

Author information

Authors and Affiliations

  1. Sol Genomics Network, Boyce Thompson Institute, Ithaca, NY, USA

    Surya Saha

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  1. Surya Saha
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Corresponding author

Correspondence to Surya Saha .

Editor information

Editors and Affiliations

  1. Division of Biology, Kansas State University, Manhattan, NY, USA

    Susan J. Brown

  2. Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA

    Michael E. Pfrender

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Saha, S. (2019). Long Range Sequencing and Validation of Insect Genome Assemblies. In: Brown, S., Pfrender, M. (eds) Insect Genomics. Methods in Molecular Biology, vol 1858. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8775-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8775-7_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8774-0

  • Online ISBN: 978-1-4939-8775-7

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