Long Range Sequencing and Validation of Insect Genome Assemblies
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Advances in long read and long range sequencing technologies have enabled chromosome length resolution for de novo genome assemblies even in the absence of complementary resources such as physical maps. Herein, I introduce a few methods for quality control and discuss potential pitfalls when assembling insect genomes with long reads.
Key wordsAssembly Scaffolding Next-generation sequencing De bruijn graph PacBio Nanopore
I would like to thank Susan Brown and Michael Pfrender for the invitation to author this article. I would also like to thank my colleagues Prashant Hosmani and Mirella Flores for insightful discussions for troubleshooting assembly issues. This work was funded by USDA NIFA grant 2015-70016-23028 funded to Susan Brown and Lukas Mueller.
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