Abstract
Isolation of high molecular weight (HMW) DNA from any organism can be daunting and populations of extremely small insects with hard exoskeletons are no exception. It can be challenging to isolate DNA that is clean enough to enzymatically label, digest or otherwise manipulate without sacrificing length. The method described here was originally developed to isolate HMW DNA for BAC library construction. More recently, it has proven reliable for long range optical mapping as well.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Zhang M, Zhang Y, Scheuring C, Wu C, Dong J, Zhang H (2012) Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research. Nat Protoc 7(3):467–478
Peterson DG, Tomkins JP, Frisch DA, Wing R, Paterson AH (2000) Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide. J Agric Genomics 5:1–100
Brown SJ, Henry JK, Black WC, Denell RE (1990) Molecular genetic manipulation of the red flour beetle: genome organization and cloning of a ribosomal protein gene. Insect Biochem 20(2):185–193
Marusyk R, Sergeant A (1980) A simple method for dialysis of small-volume samples. Anal Biochem 105:403–404
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Brown, S.J., Coleman, M. (2019). Isolation of High Molecular Weight DNA from Insects. In: Brown, S., Pfrender, M. (eds) Insect Genomics. Methods in Molecular Biology, vol 1858. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8775-7_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8775-7_3
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8774-0
Online ISBN: 978-1-4939-8775-7
eBook Packages: Springer Protocols