Isolation of High Molecular Weight DNA from Insects

  • Susan J. BrownEmail author
  • Michelle Coleman
Part of the Methods in Molecular Biology book series (MIMB, volume 1858)


Isolation of high molecular weight (HMW) DNA from any organism can be daunting and populations of extremely small insects with hard exoskeletons are no exception. It can be challenging to isolate DNA that is clean enough to enzymatically label, digest or otherwise manipulate without sacrificing length. The method described here was originally developed to isolate HMW DNA for BAC library construction. More recently, it has proven reliable for long range optical mapping as well.

Key words

High molecular weight DNA Optical mapping Long range restriction mapping 


  1. 1.
    Zhang M, Zhang Y, Scheuring C, Wu C, Dong J, Zhang H (2012) Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research. Nat Protoc 7(3):467–478CrossRefGoogle Scholar
  2. 2.
    Peterson DG, Tomkins JP, Frisch DA, Wing R, Paterson AH (2000) Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide. J Agric Genomics 5:1–100Google Scholar
  3. 3.
    Brown SJ, Henry JK, Black WC, Denell RE (1990) Molecular genetic manipulation of the red flour beetle: genome organization and cloning of a ribosomal protein gene. Insect Biochem 20(2):185–193CrossRefGoogle Scholar
  4. 4.
    Marusyk R, Sergeant A (1980) A simple method for dialysis of small-volume samples. Anal Biochem 105:403–404CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of BiologyKansas State UniversityManhattanUSA

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