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Whole-Genome Bisulfite Sequencing for the Methylation Analysis of Insect Genomes

  • Fanny Gatzmann
  • Frank LykoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1858)

Abstract

DNA methylation is a conserved epigenetic modification of animal genomes, but genome methylation patterns appear surprisingly diverse in insects. Whole-genome bisulfite sequencing (WGBS) represents a sensitive and robust method for the characterization of genome-wide methylation patterns at single-base resolution. Here, we describe a step-by-step protocol for the generation and analysis of WGBS datasets using standard Illumina sequencing platforms. In comparison to whole-genome sequencing, WGBS has additional caveats that require particular attention and are highlighted in this chapter.

Key words

Epigenetics DNA methylation detection Whole-genome bisulfite sequencing WGBS 

Notes

Acknowledgments

We thank Günter Raddatz, Stephan Wolf, Nicolle Diessl, and the DKFZ Genomics and Proteomics Core Facility for helpful discussions.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Epigenetics, DKFZ-ZMBH AllianceGerman Cancer Research CenterHeidelbergGermany

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