Arthropod Genome Sequencing and Assembly Strategies
As in any endeavor, the strategy applied to a genome project can mean the difference between success and failure. This is especially important when limited funding often means only a single approach may be tried at a given time. Although the advance of all areas of genomics and transcriptomics in recent years has led to an embarrassment of riches, methods in the field have not quite reached the turn-key production status for all species, despite being closer than ever. Here I contrast and compare the technical approaches to genome projects in the hope of enabling strategy choices with higher probabilities of success. Finally, I review the new technologies that are not yet widely distributed which are revolutionizing the future of genomics.
Key wordsGenome project strategy Genome assembly Genome sequencing Genomics Insect genomes Oxford nanopore Pacific biosciences 10× Genomics
I thank Susan Brown and Michael Pfrender for the invitation to author this article. This work was funded by NHGRI grant U54 HG003273 to Richard A. Gibbs.
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