Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development

  • Hidefumi ShinoharaEmail author
  • Yoshikatsu Matsubayashi
Part of the Methods in Molecular Biology book series (MIMB, volume 1863)


The formation of longitudinal zonation patterns is important for normal root development and is regulated by the transcription factor PLETHORA (PLT). PLT proteins form a concentration gradient, and PLT protein levels determine root zonation. A peptide hormone root meristem growth factor (RGF) and its receptors (RGFRs) act as key regulators of root development by regulating the PLT protein expression pattern. Here, we describe a method for monitoring PLT protein gradient patterns in Arabidopsis with exogenous RGF treatment.

Key words

Plant Root development Root apical meristem PLETHORA Root meristem growth factor Gradient Receptor kinases Ligand–receptor pairs 



This research was supported by a Grants-in-Aid for Scientific Research on Innovative Areas (15H05957 to Y.M., 26113520 and 16H01234 to H.S.), a Grant-in-Aid for Scientific Research (S) (25221105 to Y.M.), and a Grant-in-Aid for Young Scientists (B) (25840111 to H.S.).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Biological Science, Graduate School of ScienceNagoya UniversityNagoyaJapan

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