Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers

  • Koen Veys
  • Abdiel Alvarado-Diaz
  • Katrien De BockEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)


Endothelial cells (ECs) form the inner lining of the vascular network. Although they can remain quiescent for years, ECs exhibit high plasticity in both physiological and pathological conditions, when they need to rapidly form new blood vessels in a process called angiogenesis. EC metabolism recently emerged as an important driver of this angiogenic switch. The use of radioactive tracer substrates to assess metabolic flux rates in ECs has been essential for the discovery that fatty acid, glucose, and glutamine metabolism critically contribute to vessel sprouting. In the future, these assays will be useful as a tool for the characterization of pathological conditions in which deregulation of EC metabolism underlies and/or precedes the disease, but also for the identification of anti-angiogenic metabolic targets. This chapter describes in detail the radioactive tracer substrate assays that have been used for the determination of EC metabolic flux in vitro.

Key words

Endothelium Metabolic flux rate Radioactive tracer substrates Glycolysis Fatty acid oxidation Glucose oxidation Glutamine oxidation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Koen Veys
    • 1
    • 2
  • Abdiel Alvarado-Diaz
    • 3
  • Katrien De Bock
    • 3
    Email author
  1. 1.Laboratory of Angiogenesis and Vascular Metabolism, VIB Center for Cancer BiologyVIBLeuvenBelgium
  2. 2.Laboratory of Angiogenesis and Vascular Metabolism, Department of OncologyKU LeuvenLeuvenBelgium
  3. 3.Laboratory of Exercise and Health, Department of Health Sciences and TechnologyETH ZürichZürichSwitzerland

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