Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants

  • Wen-Rong Lie
  • Derek F. Amanatullah
  • Bonnie L. King
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)


Bone is a metabolically dynamic tissue that is continuously built up and broken down through anabolic and catabolic processes regulated by a variety of systemic and local signaling molecules. Here, we describe quantitative multiplex immunoassay analysis of supernatants collected from cultured human bone tissue fragments to profile local factors associated with the bone turnover process.

Key words

Bone turnover biomarkers Human bone tissue culture Multiplex immunoassay 



This work was supported by a grant from the METAvivor Foundation to BLK.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Wen-Rong Lie
    • 1
  • Derek F. Amanatullah
    • 2
  • Bonnie L. King
    • 3
  1. 1.MilliporeSigma CorporationSt LouisUSA
  2. 2.Department of Orthopaedic SurgeryStanford University School of MedicineRedwood CityUSA
  3. 3.Department of PediatricsStanford University School of MedicineStanfordUSA

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