Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants
Bone is a metabolically dynamic tissue that is continuously built up and broken down through anabolic and catabolic processes regulated by a variety of systemic and local signaling molecules. Here, we describe quantitative multiplex immunoassay analysis of supernatants collected from cultured human bone tissue fragments to profile local factors associated with the bone turnover process.
Key wordsBone turnover biomarkers Human bone tissue culture Multiplex immunoassay
This work was supported by a grant from the METAvivor Foundation to BLK.
- 23.Contag CH, Lie WR, Bammer MC et al (2014) Monitoring dynamic interactions between breast cancer cells and human bone tissue in a co-culture model. Molecular imaging and biology: MIB: the official publication of the academy of. Mol Imaging 16(2):158–166. https://doi.org/10.1007/s11307-013-0685-0 CrossRefGoogle Scholar