Lipectomizing Mice for Applications in Metabolism
The obesity epidemic is a critical public health problem closely associated with the development of metabolic disease. In obesity there is excess white adipose tissue, a dynamic tissue that has many biological functions. Specifically visceral adipose tissue (VAT) is an active endocrine organ producing hormones that control systemic metabolism. VAT accumulates immune cells that produce cytokines that drive chronic inflammation and promote insulin resistance. VAT can be surgically removed in experimental animals (lipectomy) to explore mechanisms by which VAT participates in metabolic, endocrine, and immunological functions. This chapter describes the technical protocol for efficient and successful removal of the gonadal fat pads in mice.
Key wordsLipectomy Visceral adipose tissue Mouse surgery Metabolism High-fat diet Obesity
Conflict of Interest Statement
The authors declare no conflict of interest.
Support for Work
National Institutes of Health grant R00 CA177868, Michigan State University start-up funds
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