Imaging Glioma Progression by Intravital Microscopy

  • Fabio StanchiEmail author
  • Ken Matsumoto
  • Holger Gerhardt
Part of the Methods in Molecular Biology book series (MIMB, volume 1862)


We describe here a method for generating mouse orthotopic gliomas in order to follow their progression over time by multi-photon laser scanning microscopy. After craniotomy of the parietal bone, glioma cells are implanted in the brain cortex and a glass window is cemented atop, allowing chronical imaging of the tumor. The expression of different fluorescent proteins in tumor cells and in specific cell types of a number of currently available transgenic mouse strains allows obtaining multicolor 3D images of the tumor over time. This technique is suitable both to evaluate the effect of pharmacological treatments and to unravel basic mechanisms of tumor-host interactions.

Key words

Intravital imaging Multi-photon Laser scanning microscopy Tumor model Glioma 



The development of the technique here described was supported by the Belgian Cancer Foundation (Stichting Tegen Kanker, grant 2012‐181) and a Hercules type 2 grant (Herculesstichting: AKUL11033). We thank Dr. Till Acker (Institute of Neuropathology, University of Giessen, Germany) and Dr. Thomas N. Seyfried (Biology department, Boston College, USA) for the gift of Glioma261 and CT2A cells, respectively.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Fabio Stanchi
    • 1
    Email author
  • Ken Matsumoto
    • 1
  • Holger Gerhardt
    • 1
    • 2
  1. 1.VIB-KU Leuven Center for Cancer Biology (CCB)LeuvenBelgium
  2. 2.Max Delbrück Center for Molecular MedicineBerlinGermany

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