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Imaging Glioma Progression by Intravital Microscopy

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Metabolic Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1862))

Abstract

We describe here a method for generating mouse orthotopic gliomas in order to follow their progression over time by multi-photon laser scanning microscopy. After craniotomy of the parietal bone, glioma cells are implanted in the brain cortex and a glass window is cemented atop, allowing chronical imaging of the tumor. The expression of different fluorescent proteins in tumor cells and in specific cell types of a number of currently available transgenic mouse strains allows obtaining multicolor 3D images of the tumor over time. This technique is suitable both to evaluate the effect of pharmacological treatments and to unravel basic mechanisms of tumor-host interactions.

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Acknowledgment

The development of the technique here described was supported by the Belgian Cancer Foundation (Stichting Tegen Kanker, grant 2012‐181) and a Hercules type 2 grant (Herculesstichting: AKUL11033). We thank Dr. Till Acker (Institute of Neuropathology, University of Giessen, Germany) and Dr. Thomas N. Seyfried (Biology department, Boston College, USA) for the gift of Glioma261 and CT2A cells, respectively.

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Authors and Affiliations

  1. VIB-KU Leuven Center for Cancer Biology (CCB), Leuven, Belgium

    Fabio Stanchi, Ken Matsumoto & Holger Gerhardt

  2. Max Delbrück Center for Molecular Medicine, Berlin, Germany

    Holger Gerhardt

Authors
  1. Fabio Stanchi
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  2. Ken Matsumoto
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  3. Holger Gerhardt
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Corresponding author

Correspondence to Fabio Stanchi .

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Editors and Affiliations

  1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium

    Sarah-Maria Fendt

  2. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA

    Sophia Y. Lunt

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Stanchi, F., Matsumoto, K., Gerhardt, H. (2019). Imaging Glioma Progression by Intravital Microscopy. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8769-6_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8768-9

  • Online ISBN: 978-1-4939-8769-6

  • eBook Packages: Springer Protocols

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