Abstract
Somatic cell nuclear transfer (SCNT) enables the production of animals from single cell nuclei. Unlike normally fertilized embryos, SCNT-derived embryos ectopically express the Xist gene from the maternal allele, because of the lack of Xist-repressing imprints in the somatic donor genome. This has severely compromised the development of SCNT-derived embryos, at least in mice. Here, we describe the basic protocol of mouse SCNT as well as a Xist knockdown SCNT procedure, which remarkably increases the efficiency of cloning mice.
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Inoue, K., Matoba, S., Ogura, A. (2018). Somatic Cell Nuclear Transfer in Mice: Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_5
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DOI: https://doi.org/10.1007/978-1-4939-8766-5_5
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