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Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation

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X-Chromosome Inactivation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1861))

Abstract

X-chromosome inactivation is a dosage compensation mechanism that equalizes X-linked gene expression between male and female mammals through the transcriptional silencing of most genes on one of the two X-chromosomes in females. With a few key exceptions, once the X-chromosome is inactivated replicated copies of that X-chromosome are maintained as inactive in all descendant cells. X-inactivation is therefore a paradigm of epigenetic inheritance. Imprinted X-inactivation is a specialized form of X-inactivation that results in the silencing of the paternally derived X-chromosome. Due to its parent-of-origin-specific pattern of inactivation, imprinted X-inactivation is a model of mitotic as well as meiotic, i.e., transgenerational, epigenetic inheritance. All cells of the early mouse embryo undergo imprinted X-inactivation, a pattern that is subsequently maintained in extraembryonic cell types in vivo and in vitro. Here, we describe both high- and low-throughput approaches to interrogate imprinted X-inactivation in the mouse embryo as well in cultured extraembryonic stem cells.

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Acknowledgments

This work was funded by an NIH training grant: Michigan Predoctoral Training in Genetics (T32GM007544) to M.C.; NIH National Research Service Award #5-T32-GM07544 from the National Institute of General Medicine Sciences to E.M.; an NIH Director’s New Innovator Award (DP2-OD-008646-01) to S.K.; a March of Dimes Basil O’Connor Starter Scholar Research Award (5-FY12-119); and the University of Michigan Endowment for Basic Sciences.

Author information

Author notes

  1. Emily Maclary

    Present address: Department of Biology, University of Utah, Salt Lake City, UT, USA

  2. Marissa Cloutier, Clair Harris, and Srimonta Gayen contributed equally to this work.

Authors and Affiliations

  1. Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA

    Marissa Cloutier, Clair Harris, Srimonta Gayen, Emily Maclary & Sundeep Kalantry

Authors
  1. Marissa Cloutier
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  2. Clair Harris
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  3. Srimonta Gayen
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  4. Emily Maclary
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  5. Sundeep Kalantry
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Corresponding author

Correspondence to Sundeep Kalantry .

Editor information

Editors and Affiliations

  1. Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan

    Takashi Sado

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Cloutier, M., Harris, C., Gayen, S., Maclary, E., Kalantry, S. (2018). Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8766-5_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8765-8

  • Online ISBN: 978-1-4939-8766-5

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