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X-Chromosome Inactivation pp 1–18Cite as

Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1861))

Abstract

Forward genetics can provide insight into molecular pathways as has been demonstrated by advances in cell biology from comprehensive genetic studies in simple organisms. Recently, techniques have become available that promise a similar potential for understanding developmental pathways in mammals. Here we describe a genetic forward screening approach for identifying factors involved in X chromosome inactivation that is based on haploid mouse embryonic stem cells. Using a genetically encoded selection system screening of large mutant pools can identify candidate silencing factors with high confidence.

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Abbreviations

ESC:

Embryonic stem cell

PBS:

Phosphate buffered saline

RT:

Room temperature

TX100:

Triton X-100

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Acknowledgment

This work received support from the Swiss National Science Fond (grants 31003A_152814 and 316030_145026). GDM was supported by an ETH Postdoctoral Fellowship. We are grateful to T. Brummelkamp for reagents and advice for the viral gene trap mutagenesis and insertion identification.

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Authors and Affiliations

  1. Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, ETH Hönggerberg, Zurich, Switzerland

    Asun Monfort, Giulio Di Minin & Anton Wutz

Authors
  1. Asun Monfort
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  2. Giulio Di Minin
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  3. Anton Wutz
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Corresponding authors

Correspondence to Asun Monfort or Anton Wutz .

Editor information

Editors and Affiliations

  1. Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan

    Takashi Sado

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Monfort, A., Di Minin, G., Wutz, A. (2018). Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8766-5_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8765-8

  • Online ISBN: 978-1-4939-8766-5

  • eBook Packages: Springer Protocols

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