SNAREs pp 391-401 | Cite as

Imaging SNAP-29 in Drosophila

  • Hao XuEmail author
  • Bryan Stewart
Part of the Methods in Molecular Biology book series (MIMB, volume 1860)


SNAP-29 is expressed throughout the life cycle of fruit fly and exhibits wide tissue distribution patterns. Unlike other SNAP-25-like proteins (i.e., SNAP-25, SNAP-23/24, and SNAP-47) which primarily support exocytosis at the plasma membrane, SNAP-29 regulates various intracellular trafficking events, by partnering with proteins active in both exocytosis and endocytosis. Here we describe the protocol to localize SNAP-29 in early embryos, imaginal discs from third instar larva, and immortalized S2 cells via immunofluorescence microscopy.

Key words

SNAP-29 Ubisnap usnp Drosophila 



We thank Dr. Julie Brill for critical inputs.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Biological, Environmental, and Earth SciencesUniversity of Southern MississippiHattiesburgUSA
  2. 2.Department of BiologyUniversity of Toronto MississaugaMississaugaCanada

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