Abstract
Necroptosis in macrophages is increasingly being recognized as a significant contributor to the inflammation seen in many pathologies. Here we describe a well-known method to obtain quiescent, mature macrophages that can be used to study the molecular mechanisms that regulate necroptosis. We also describe two ways to quantify this form of programmed cell death.
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Acknowledgments
This work was supported by grants AI052417 and DK072201 from the NIH, and a Senior Research Award from the Crohn’s and Colitis Foundation of America (CCFA).
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Legarda, D., Ting, A.T. (2018). Analysis of Necroptosis in Bone Marrow-Derived Macrophages. In: Ting, A. (eds) Programmed Necrosis. Methods in Molecular Biology, vol 1857. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8754-2_6
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DOI: https://doi.org/10.1007/978-1-4939-8754-2_6
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