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Programmed Necrosis pp 19–33Cite as

Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1857))

Abstract

Necroptosis is mediated by the formation of the detergent-insoluble necrosome complex between Ser/Thr kinases RIPK1 and RIPK3, which mediates RIPK3-dependent phosphorylation and activation of the critical necroptosis effector MLKL. Small molecule screens have been instrumental in the development of new chemical probes for this pathway. In this chapter, we describe several cellular assays that are readily amendable for the identification of new modulators of necroptosis as well as secondary assays to facilitate initial characterization of the mode of activity of small molecule hits.

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Authors and Affiliations

  1. Medical Scientist Training Program, Program in Neuroscience, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA, USA

    Danish Saleh

  2. Department of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA, USA

    Alexei Degterev

Authors
  1. Danish Saleh
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  2. Alexei Degterev
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Corresponding author

Correspondence to Alexei Degterev .

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Editors and Affiliations

  1. Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA

    Adrian T. Ting

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Saleh, D., Degterev, A. (2018). Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis. In: Ting, A. (eds) Programmed Necrosis. Methods in Molecular Biology, vol 1857. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8754-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8754-2_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8753-5

  • Online ISBN: 978-1-4939-8754-2

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