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Radiolabeling and Analysis of Labeled Proteins

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Protein Gel Detection and Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

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Abstract

Protein-based preparations for biomedical research, and radiolabeled pharmaceuticals for nuclear medical diagnostics and therapy require highest chemical, radiochemical, and in particular protein purity. For detection of contaminations most commonly size exclusion high-performance liquid chromatography and polyacrylamide gel analysis in combination with silver staining is used. Here we describe a by far more sensitive radiolabeling method for the detection of traces of contaminating proteins.

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References

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Authors and Affiliations

  1. Institute of Radiopharmaceutical Cancer Research, Radioimmunology, Helmholtz-Zentrum Dresden-Rossendorf e.V.(HZDR), Dresden, Germany

    Nicole Berndt, Ralf Bergmann & Michael Bachmann

Authors
  1. Nicole Berndt
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  2. Ralf Bergmann
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  3. Michael Bachmann
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Corresponding author

Correspondence to Michael Bachmann .

Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Berndt, N., Bergmann, R., Bachmann, M. (2018). Radiolabeling and Analysis of Labeled Proteins. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_33

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  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_33

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

  • eBook Packages: Springer Protocols

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